georefid:2003-021712SEDIS Publication Catalogueana.macario@awi.dehttp://sedis.iodp.org/pub-catalogue/informationpointOfContact2011-06-23T00:00:00Zhttp://sedis.iodp.org/pub-catalogue/index.php?id=10.1126/science.1075653<gco:CharacterString>Fluids from aging ocean crust that support microbial life</gco:CharacterString>2003-01-01publicationgeorefid:2003-021712doi:10.1126/science.1075653Cowen, James P.University of Hawaii, Department of Oceanography, Honolulu, HI, United StatesauthorGiovannoni, Stephen J.Oregon State University, United StatesauthorKenig, FabienUniversity of Illinois at Chicago, United StatesauthorJohnson, H. PaulUniversity of Washington, United StatesauthorButterfield, DavidauthorRappe, Michael S.authorHutnak, MichaelauthorLam, PhyllisauthorAmerican Association for the Advancement of Science, Washington, DC, United StatespublisherdocumentDigitalScience299 (5603)120-123Little is known about the potential for life in the vast, low-temperature (<100 degrees C) reservoir of fluids within mid-ocean ridge flank and ocean basin crust. Recently, an overpressured 300-meter-deep borehole was fitted with an experimental seal (CORK) delivering crustal fluids to the sea floor for discrete and large-volume sampling and characterization. Results demonstrate that the 65 degrees C fluids from 3.5-million-year-old ocean crust support microbial growth. Ribosomal RNA gene sequence data indicate the presence of diverse Bacteria and Archaea, including gene clones of varying degrees of relatedness to known nitrate reducers (with ammonia production), thermophilic sulfate reducers, and thermophilic fermentative heterotrophs, all consistent with fluid chemistry.completedHydrochemistryOceanographyArchaeabacteriaboreholescrustdeep-sea environmentEast Pacificecologygeochemistryhydrocarbonshydrochemistryhydrothermal conditionsinstrumentsJuan de Fuca RidgeLeg 168low temperaturemarine environmentmicroorganismsmid-ocean ridgesNorth PacificNortheast PacificOcean Drilling Programocean floorsoceanic crustODP Site 1026organic compoundsPacific OceanRNAsea watertemperaturethermophilic taxaurn:org.iodp:exp:168urnlargerWorkCitationcampaignurn:org.iodp:exp:168:site:1026urnlargerWorkCitationstudyurn:org.iodp:exp:168urnlargerWorkCitationcampaignEnglishgeoscientificInformation-127.4526-127.452647.455647.4556application/pdfunknownSEDIS Publication Catalogueana.macario@awi.dehttp://sedis.iodp.org/pub-catalogue/informationpointOfContacthttp://www.sciencemag.org/content/299/5603/120.full.pdfdownload