georefid:2012-014432georefid:2012-014422SEDIS Publication Catalogueana.macario@awi.dehttp://sedis.iodp.org/pub-catalogue/informationpointOfContact2013-07-08T00:00:00Zhttp://sedis.iodp.org/pub-catalogue/index.php?id=10.2204/iodp.proc.329.110.2011<gco:CharacterString>Preliminary experiment for cell count using flow cytometry</gco:CharacterString>2011-01-01publicationgeorefid:2012-014432doi:10.2204/iodp.proc.329.110.2011Morono, YukiJapan Agency for Marine-Earth Science and Technology, Kochi Institute for Core Sample Research, Kochi, JapanauthorKallmeyer, JensJapan Agency for Marine-Earth Science and Technology, JapanauthorInagaki, FumioIntegrated Ocean Drilling Program, United StatesauthorD'Hondt, StevenLamont-Doherty Earth Observatory, United StatesauthorAlvarez Zarikian, CarlosDalhousie University, CanadaauthorEvans, HelenCarl von Ossietzky Universitaet Oldenburg, GermanyauthorDubois, NathalieMax-Planck-Institute of Marine Microbiology, GermanyauthorEngelhardt, TimAarhus Universitet, DenmarkauthorFerdelman, TimothyOregon State University, United StatesauthorGribsholt, BrittaMinnesota State University-Mankato, United StatesauthorHarris, Robert N.Hanyang University, South KoreaauthorHoppie, Bryce W.University of Potsdam, GermanyauthorHyun, Jung-HoYonsei University, South KoreaauthorKim, JinwookUniversity of Melbourne, AustraliaauthorLynch, Jill E.Shizuoka University, JapanauthorMitsunobu, SatoshiBoston University, United StatesauthorMurray, Richard W.University of Tsukuba, JapanauthorReese, Brandi K.Kyushu University, JapanauthorShimono, TakayaHiroshima University, JapanauthorShiraishi, FumitoUniversity of Southampton, United KingdomauthorSmith, David C.University of Bergen, NorwayauthorSmith-Duque, Christopher E.AIST/Geological Survey of Japan, JapanauthorSpivack, Arthur J.Dublin City University, IrelandauthorSteinsbu, Bjorn OlavIFREMER, FranceauthorSuzuki, YoheyChiba University, JapanauthorSzpak, MichalUniversity of Tokyo, JapanauthorToffin, LaurentChinese Academy of Sciencesa, South China Sea Institute of Oceanology, ChinaauthorUramoto, GoichiroOcean University of China, ChinaauthorYamaguchi, YasuhikoUniversity of Southern California, United StatesauthorZhang GuoliangUniversity of Potsdam, GermanyauthorZhang XiaohuaUniversity of Rhode Island, United StatesauthorZiebis, WiebkeIntegrated Ocean Drilling Program, United StatesauthorIODP Management International, Washington, DC, United StatespublisherdocumentDigitalIn: D'Hondt, Steven, Inagaki, Fumio, Alvarez Zarikian, Carlos, Evans, Helen, Dubois, Nathalie, Engelhardt, Tim, Ferdelman, Timothy, Gribsholt, Britta, Harris, Robert N., Hoppie, Bryce W., Hyun, Jung-Ho, Kallmeyer, Jens, Kim, Jinwook, Lynch, Jill E., Mitsunobu, Satoshi, Morono, Yuki, Murray, Richard W., Reese, Brandi K., Shimono, Takaya, Shiraishi, Fumito, Smith, David C., Smith-Duque, Christopher E., Spivack, Arthur J., Steinsbu, Bjorn Olav, Suzuki, Yohey, Szpak, Michal, Toffin, Laurent, Uramoto, Goichiro, Yamaguchi, Yasuhiko, Zhang Guoliang, Zhang Xiaohua, Ziebis, Wiebke, Proceedings of the Integrated Ocean Drilling Program; South Pacific gyre subseafloor; Expedition 329 of the riserless drilling platform; Papeete, Tahiti, to Auckland, New Zealand Sites U1365-U1371, 9 October-13 December 2010332One of the major challenges in microbial ecology is to evaluate the accurate number of living cells in a natural environment. Cell count using flow cytometry is a powerful, high-throughput technique that has widely been used for aquatic habitats but not for sedimentary environments because mineral grains interfere with cell detection. During Integrated Ocean Drilling Program Expedition 329, we tested several sample preparation methods for onboard cell counting using flow cytometry with various pelagic sediments from the South Pacific Gyre. The cell numbers acquired from shallow sediments are almost consistent with microscopic direct counts (i.e., approximately 105 cells/cm (super 3) ). Yet, the method needs improvement and standardization to reduce the background signal and to lower the minimum detection limit for deep sedimentary habitats with very low cell densities (i.e., <1000 cells/cm (super 3) ).completedOceanographyboreholescoresdetectionecologyExpedition 329experimental studiesflow cytometrygeomicrobiologyIntegrated Ocean Drilling ProgramIODP Site U1365IODP Site U1366IODP Site U1367IODP Site U1368IODP Site U1369IODP Site U1370IODP Site U1371laboratory studiesmarine sedimentsmethodsmicroorganismsPacific OceansedimentsSouth Pacificurn:org.iodp:exp:329urnlargerWorkCitationcampaignurn:org.iodp:exp:329:site:U1365urnlargerWorkCitationstudyurn:org.iodp:exp:329urnlargerWorkCitationcampaignurn:org.iodp:exp:329:site:U1366urnlargerWorkCitationstudyurn:org.iodp:exp:329urnlargerWorkCitationcampaignurn:org.iodp:exp:329:site:U1367urnlargerWorkCitationstudyurn:org.iodp:exp:329urnlargerWorkCitationcampaignurn:org.iodp:exp:329:site:U1368urnlargerWorkCitationstudyurn:org.iodp:exp:329urnlargerWorkCitationcampaignurn:org.iodp:exp:329:site:U1369urnlargerWorkCitationstudyurn:org.iodp:exp:329urnlargerWorkCitationcampaignurn:org.iodp:exp:329:site:U1370urnlargerWorkCitationstudyurn:org.iodp:exp:329urnlargerWorkCitationcampaignurn:org.iodp:exp:329:site:U1371urnlargerWorkCitationstudyurn:org.iodp:exp:329urnlargerWorkCitationcampaignEnglishgeoscientificInformation-165.3900-123.0900-45.5800-23.5100application/pdfunknownSEDIS Publication Catalogueana.macario@awi.dehttp://sedis.iodp.org/pub-catalogue/informationpointOfContacthttp://publications.iodp.org/proceedings/329/EXP_REPT/CHAPTERS/329_110.PDFdownload